FDNB staining of proteins separated by disc electrophoresis
β Scribed by Nicholas Catsimpoolas
- Book ID
- 107710824
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 180 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A fast and convenient method for silver staining of proteins on electroblotting membranes was developed based on Gallyas' histochemical intensifier and applied to human endothelial cell proteins separated by one-and two-dimensional electrophoresis and electroblotted to polyvinyl difluoride membranes
Staining of sodium dodecyl sulfate or lithium dodecyl sulfate gels with 3,3',5,5'-tetramethylbenzidine (TMBZ)/H2O2 after electrophoresis has frequently been used as a specific method of detecting heme proteins. That TMBZ is an electron donor for O2 reduction by the nonheme-soluble cytochrome oxidase