✦ LIBER ✦
3,3′,5,5′-Tetramethylbenzidine/H2O2 staining is not specific for heme proteins separated by gel electrophoresis
✍ Scribed by David J. Miller; D.J.D. Nicholas
- Book ID
- 102627600
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 274 KB
- Volume
- 140
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Staining of sodium dodecyl sulfate or lithium dodecyl sulfate gels with 3,3',5,5'-tetramethylbenzidine (TMBZ)/H2O2 after electrophoresis has frequently been used as a specific method of detecting heme proteins. That TMBZ is an electron donor for O2 reduction by the nonheme-soluble cytochrome oxidase/nitrite reductase from Nitrosomonas europaea is now shown; this protein is detected by the TMBZ/H2O2 method. A method for the determination of TMBZ oxidase activity is given; hence, the detection of artifactual staining due to proteins of this type is possible.