✦ LIBER ✦

Fatty acid composition of phospholipids in epithelium and stroma of human benign prostatic hyperplasia

✍ Scribed by Weisser, H.; Krieg, M.

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 294 KB
Volume: 36
Category: Article
ISSN: 0270-4137
DOI: 10.1002/(sici)1097-0045(19980901)36:4<235::aid-pros4>3.0.co;2-e

No coin nor oath required. For personal study only.

✦ Synopsis

BACKGROUND.

Although it is well known that prostatic 5␣-reductase is active only in its membrane-bound form, rather limited information is available concerning the composition of cellular lipids in human BPH. Therefore, in the present study, the phospholipid fatty acid composition and content in epithelium and stroma of human BPH have been investigated for the first time. METHODS. Phospholipids separated on TLC plates were methylated and fatty acid methyl esters were analyzed by capillary gas chromatography. RESULTS. The fatty acid composition of total phospholipids was significantly different between epithelium and stroma. In particular, the percentage of oleic acid was significantly higher in epithelium as compared with stroma, whereas that of arachidonic acid was significantly lower in epithelium than in stroma. In addition, significant differences between epithelium and stroma were found in regard to the fatty acid composition of the main phospholipid subclasses. Another remarkable finding were the age-dependent changes of the fatty acid composition in human BPH. CONCLUSIONS. This study shows that the fatty acid composition of phospholipids is significantly different between epithelium and stroma of human BPH. Furthermore, agedependent alterations of the fatty acid composition were found. Further studies are needed to determine whether the endogenous hormonal milieu in the prostate modulates the fatty acid composition of the prostatic cells, as well as what impact such modulation could have on the properties of membrane proteins, i.e., enzymes like the 5␣-reductase and receptors, which are thought to be affected by alterations in membrane fluidity or composition, or both.

📜 SIMILAR VOLUMES

Lipid composition in epithelium and stro

Lipid composition in epithelium and stroma of human benign prostatic hyperplasia

✍ Weisser, Heike; Krieg, Michael 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 245 KB 👁 2 views

The lipid composition and concentration in human benign prostatic hyperplasia (BPH) were investigated. The reason was to shed some light onto the lipid environment of cellular membranes, in which the epithelial and stromal 5␣-reductase of the human prostate have apparently to be embedded in order to

Prediagnostic level of fatty acids in se

Prediagnostic level of fatty acids in serum phospholipids: Ω-3 and Ω-6 fatty acids and the risk of prostate cancer

✍ Sverre Harvei; Kristian S. Bjerve; Steinar Tretli; Egil Jellum; Trude E. Robsahm 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 51 KB 👁 1 views

Ecological and case-control studies have demonstrated a positive correlation between consumption of fat and the risk of prostate cancer. Two recent human studies have focused on a-linolenic acid as a risk factor for prostate cancer. Animal experiments have shown that dietary v-6 polyunsaturated fatt

Influence of different fats in pig feed

Influence of different fats in pig feed on fatty acid composition of phospholipids and physical meat quality characteristics

✍ Martin R. L. Scheeder; Karola R. Gläser; Barbara Eichenberger; Caspar Wenk 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 74 KB 👁 1 views

Fatty-acid composition in serum phosphol

Fatty-acid composition in serum phospholipids and risk of breast cancer: An incident case-control study in Sweden

✍ Véronique Chajès; Kerstin Hultén; Anne-Linda Van Kappel; Anna Winkvist; Rudolf K 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 French ⚖ 100 KB 👁 2 views

The study of the relationship between dietary intake of fatty acids and the risk of breast cancer has not yielded definite conclusions with respect to causality, possibly because of methodological issues inherent to nutritional epidemiology. To evaluate the hypothesis of possible protection of n-3 p

Oxidation of muscle phospholipids in rel

Oxidation of muscle phospholipids in relation to their fatty acid composition with emphasis on volatile compounds

✍ Meynier, A; Genot, C; Gandemer, G 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 107 KB 👁 1 views

The impact of moderate changes of fatty acid (FA) composition of phospholipids (PL) on their oxidative stability and on volatile pro®les remains largely unknown. PL of breast muscle of turkeys fed a diet containing 6% tallow, rapeseed oil or soya oil were puri®ed and prepared as liposomes. After 24

Piperidine ester derivatives in mass spe

Piperidine ester derivatives in mass spectrometric analysis of fatty acids from human serum phospholipids and cholesteryl esters

✍ Kirsi-Marja Marnela; Teemu Moilanen; Mirjami Jutila 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 235 KB 👁 1 views

When fatty acids with unknown structural features are confronted in mass spectral analyses of complex mixtures the cleavage pattern from the widely used methyl ester derivatives is not always sufficiently informative. Their mass spectra often contain only weak signals for the parent or any other cha