Fatty acid composition of lipids of Escherichia coli W 1655 F+ and its stable protoplast type L-form

✍ Scribed by Dr. J. Gumpert; W. Schade; D. Krebs; S. Baykousheva; E. Ivanova; As. Toshkov

• Book ID: 102392535
• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 356 KB
• Volume: 22
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.19820220304

✦ Synopsis

Abstract

The comparative fatty acid analysis of extractable and non‐extractable lipids of Escherichia coli W 1655 F^+^ and its stable protoplast type L‐form shows quantitative as well as qualitative differences. From 10 different fatty acids obtained 16:0, 17:0, and 18:0 are present at about the same quantities in the lipid fractions of the bacterial and L‐form. The absence of larger amounts of 12:0, 14:0, and 14:βOH fatty acids in non‐extractable L‐form lipids reflects the loss of the cell wall in L‐form cells. 16:1 fatty acid was found in L‐form lipids only. This qualitative difference and the 2–3 times higher content of 18:1 in L‐form lipids and the 7 times lower content of cyc 19:0 in extractable lipids of the L‐form may be interpreted as alterations characteristic for the changed composition of the cytoplasmic membrane in L‐form cells.