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Fate of [G-3H]glutamate and [U-14C]glucose in the rat liver in vivo

โœ Scribed by M.K. Gaitonde; Richard D. Churchill; Gwyneth Evans


Book ID
107770170
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
334 KB
Volume
12
Category
Article
ISSN
0020-711X

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A method is described for measuring the activity of glucose-6-phosphatase (EC 3.1.3.9) in rat liver. [U-W]Glucose 6-phosphate, as substrate, is converted by the enzyme to [Ylglucose and inorganic phosphate. The addition of ZnSO, and Ba(OH), at the end of the reaction precipitates phosphate and the u