Fast ferritin immunoassay on PDMS microchips

✍ Scribed by Walter Schrott; Marek Nebyla; Lucie Meisterová; Michal Přibyl

• Book ID: 111491415
• Publisher: Versita
• Year: 2011
• Tongue: English
• Weight: 214 KB
• Volume: 65
• Category: Article
• ISSN: 0366-6352
• DOI: 10.2478/s11696-010-0079-6

✦ Synopsis

Abstract

A heterogeneous sandwich immunoassay of ferritin on a poly(dimethylsiloxane) microfluidic chip is proposed. An undemanding “prepolymerization technique” based on wet treatment of a phosphor bronze substrate was used for the microchip fabrication. Receptor rabbit antibodies were immobilized via passive sorption directly on microchannel walls. After the incubation of ferritin samples, secondary biotinylated antibodies were introduced. A solution of avidin molecules labeled by fluorescein isothiocyanate was finally added into the microchannels. Lamp-based fluorescence detection of the immunocomplex was then carried out. Dynamic detection range of the method was in the interval from 100 ng mL−1 to 10 μg mL−1.