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Fast-atom-bombardment mass spectrometry of sulphated oligosaccharides from ovine lutropin

✍ Scribed by Anne Dell; Howard R. Morris; Fiona Greer; Joanne M. Redfern; Mark E. Rogers; Gerhard Weisshaar; Jun Hiyama; Alistair G.C. Renwick

Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 979 KB
Volume: 209
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(91)80143-b

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✦ Synopsis

The positive- and negative-ion f.a.b.-mass spectra and the fragmentation of sulphated oligosaccharides derived from ovine lutropin are described. Negative-ion f.a.b.-m.s. of methylated derivatives offers a sensitive and rapid method for screening glycans for sulphation, for defining the location of sulphated residues, and for sequencing sulphated branches. Positive-ion f.a.b.-m.s. gives complementary data on non-sulphated branches in both complex and hybrid-type sulphated structures.

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