✍ Scribed by Anne Dell; Howard R. Morris; Heinz Egge; Hubertus von Nicolai; Gérard Strecker
No coin nor oath required. For personal study only.
The potential of fast-atom-bombardment (f.a.b.) mass-spectrometry in the carbohydrate field was assessed with the aid of unmodified, permethylated, and peracetylated oligosaccharides and glycosphingolipids. F.a.b. spectra are presented for (o-Gal--+&GlcNAc),+(D-Man),-+D-GlcNAc, permethylated (D-Gal+D-GlcNAc), -+(o-Man),-n-[2H]GlcNAcol, permethylated gangliotetraosylceramide, and reduced and peracetylated tetra-to hepta-saccharides from human milk. From unmodified oligosaccharides, pseudomolecular ions (M + Na+) were obtained as major ions in the high-mass range. Permethylated oligosaccharides and glycosphingolipids gave pseudomolecular ions (M + H+) of high intensity, together with fragment ions of high diagnostic importance. At ambient temperature, f.a.b. spectra could only be obtained for the lower homologs of per-0-acetylated oligosaccharides. Reduced and peracetylated penta-to hepta-saccharides from human milk gave f.a.b. spectra only after heating of the target.
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