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Fast analysis of prominent flavonoids in tomato using a monolithic column and isocratic HPLC

✍ Scribed by Magdalena Biesaga; Urszula Ochnik; Krystyna Pyrzynska

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
654 KB
Volume
32
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

RP HPLC–UV–MS was optimized for simultaneous determination of several prominent flavonoids (quercetin, naringenin, naringin, myricetin, rutin and kaempferol) using a commercially available monolithic column and isocratic elution. The concentration of organic modifier (methanol and ACN) was tested in order to obtain the best resolution. The LODs for a UV detector at 254 nm were comprised between 0.1 μg/mL for myricetin and quercetin and 0.5 μg/mL for rutin, naringin and kaempferol with the precision in the range of 1.5–3.0% RSD. MS detection with multiply reaction monitoring (MRM) offers a considerable gain in selectivity as well as sensitivity LODs for flavonoids were in the range of 1–20 ng/mL. The developed method was used for monitoring the content of some flavonoids in tomatoes during their maturation process.

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