Direct Injection Analysis of Chlorzoxazone and its Major Metabolite 6-Hydroxychlorzoxazone in Human Serum using a Semipermeable Surface (SPS) HPLC Column
✍ Scribed by A. Haque; J. T. Stewart
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 69 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0269-3879
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✦ Synopsis
A semipermeable surface (SPS) HPLC column has been used to develop a direct injection method for the simultaneous determination of chlorzoxazone and its 6-hydroxy metabolite in serum. Spiked serum was filtered and 20 L injected directly onto the SPS column. The separation was achieved within 25 min using a mobile phase of 20:80 v/v acetonitrile-aqueous 0.05 M phosphate buffer pH 7 with detection at 280 nm. The method was linear in the concentration range 0.5-20 g/mL (r > 0.999, n = 6) with an RSD < 5%. The limits of quantitation and detection were 0.5 g/mL and 0.1 g/mL (S/N > 3), respectively, for both drug and metabolite. Intraday and interday reproducibility was р 6% with recoveries of drug and metabolite > 94%.