Fast amperometric immunoassay for hantavirus infection

✍ Scribed by Rajesh Krishnan; Andrey L. Ghindilis; Plamen Atanasov; Ebtisam Wilkins; Jim Montoya; Frederick T. Koster

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 597 KB
• Volume: 8
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.1140081211

✦ Synopsis

The determination of specific antibodies against Hantavirus in blood plasma has been developed. The assay technique is based on a flow through amperometric immunoelectrode. Highly dispersed carbon material serves as an immunosorbent and at the same time as an electrode material. The analysis employed the 'sandwich' assay scheme. Recombinant protein of Hantavirus was immobilized on the surface of the immunosorbent. Target analyte captured by the immunosorbent at the first stage of incubation interacts with the peroxidase labeled antihuman antibodies at the second stage of incubation. The detection of peroxidase label was conducted amperometrically by electroreduction of iodine formed as a product of peroxidase catalyzed reaction. The technique permits determination of specific antibodies against Hantavirus in blood plasma using a 22min assay time.