Failure of centromere separation leads to formation of diplochromosomes in next mitosis in okadaic acid treated HeLa cells.

✍ Scribed by Sibdas Ghosh; Neidhard Paweletz; Dieter Schroeter

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 260 KB
• Volume: 17
• Category: Article
• ISSN: 1065-6995
• DOI: 10.1006/cbir.1993.1018

✦ Synopsis

Abstract

High concentrations of okadaic acid, sufficient to inhibit phosphatase 1 and 2A activities, induces formation of diplochromosomes in HeLa cells. It has been shown that this is due to a failure of sister chromatid separation in earlier mitosis in the presence of okadaic acid in the medium and not due to bypassing of mitosis (endoreduplication). Moreover, it has been demonstrated that the sister chromatid adherence does not depend on any under‐replicated chromatin segment shared by the sister chromatids which might happen in okadaic acid induced premature mitosis, but due to the failure of the centromeres to separate at metaphase ‐ anaphase transition. The role of phophatase 1 in sister chromatid separation has been discussed