Factors influencing the incidence of habituation for cytokinin of tobacco pith tissue in culture

✍ Scribed by Frederick Meins; Joseph Lutz; Rachel Foster

• Publisher: Springer-Verlag
• Year: 1980
• Tongue: English
• Weight: 475 KB
• Volume: 150
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00390836

✦ Synopsis

Pith tissue ofNicotiana tabacum L. cv. "Havana 425" exhibits a gradient in its tendency to habituate for cytokinin on an auxin-containing medium at 35 ~ C, about 10 ~ C above the standard culture temperature. Explants of pith from below the 8th to 1 lth internode, counting from the bottom of the plant, rarely habituate for cytokinin; explants from above this threshold habituate rapidly. The explants must also be above a critical size, about 20-30 rag, to habituate. There was a pronounced interaction between size and position effects; the threshold position for cytokinin habituation shifted upward with decreasing explant size.