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Factors influencing the chromatographic analysis of 2,2′-O-cyclocytidine

✍ Scribed by R.L. Furner; J.D. Strobel; S.M. El Dareer; L.B. Mellett

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
493 KB
Volume
92
Category
Article
ISSN
1873-3778

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✦ Synopsis

The analysis of cyclocytidine using radioactive tracer techniques coupled with instant thin-layer chromatography depends upon maintaining the stability of the compound throughout the analysis. At pH 7.0 or below cyclocytidine is stable in phosphate, citrate, and formate buffer, but the addition of heavy metal ions to these buffers may yield unstable conditions.

The data emphasize the need for carefully controlled conditions in the analysis of cyclocytidine.

In general, the data are in agreement with previous reports in that cyclocytidine is hydrolyzed to arabinosylcytosine in alkaline solutions. Based upon the experiments reported herein, we suggest that the hydrolysis of cyclocytidine is non-enzymatic, and that a number of factors related to sample handling and chromatographic conditions will influence the rate of hydrolysis.

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