𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Factors affecting the production of glutamine in cultured mouse cells

✍ Scribed by Paul R. Barnes; Dean Youngberg; Paul A. Kitos

Publisher
John Wiley and Sons
Year
1971
Tongue
English
Weight
808 KB
Volume
77
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Glutamine synthetase activity of NCTC clone 929 mouse cells (strain L) was studied as a function of the prior nutritional experience of the cells. Small enzyme increases were recorded in response to either glutamine depletion or chronic serum supplementation of the growth medium. Somewhat greater increases resulted from the administration of cortisol or certain other steroids, particularly if the hormone treatment was combined with glutamine withdrawal. High concentrations of glutamate in the medium did not augment the glutamine synthetase content of the cells and even caused an apparent decrease in it. The presence of glutamine in the culture medium resulted in a fairly rapid rate of disappearance of the glutamine synthetase of previously induced cells. The data suggest that glutamine and cortisol act independently on the cells in regulating the level of the enzyme.

πŸ“œ SIMILAR VOLUMES

Murine sarcoma growth factors affect the
Murine sarcoma growth factors affect the growth and morphology of cultured mouse epithelial cells
✍ Jorma Keski-Oja; Joseph E. De Larco; Ulf R. Rapp; George J. Todaro πŸ“‚ Article πŸ“… 1980 πŸ› John Wiley and Sons 🌐 English βš– 570 KB

## Abstract A mouse embryo epithelial cell line, MMC‐E, was used to study the effects of purified murine sarcoma growth factors (SGFs) on the growth of epithelial cells. Murine SGF was a partially purified preparation from the serum‐free culture media of mouse fibroblasts transformed by Moloney mur

Characterization of factor(s) in culture
Characterization of factor(s) in culture supernatants affecting cell social behavior
✍ James A. Weston; Kenneth M. Yamada; Karen L. Hendricks πŸ“‚ Article πŸ“… 1979 πŸ› John Wiley and Sons 🌐 English βš– 829 KB

## Abstract Treatment of embryonic chick heart fibroblast cultures with 0.2 M urea reversibly increases cellular overlap. The increase in cellular overlapping over that in control cultures may be quantitated by the overlap ratio (R), the ratio of the number of superimposed nuclei observed, to the n

Factors affecting the finding of cancer
Factors affecting the finding of cancer cells in the blood
✍ R. A. Sellwood; S. W. A. Kuper; P. M. Payne; J. Ian Burn πŸ“‚ Article πŸ“… 1969 πŸ› John Wiley and Sons 🌐 English βš– 391 KB

CANCER CELLS IN BLOOD 649 reduction of wound infection, but reasons are given for believing that compared with improvements in air cleanliness these are relatively insignificant. ## 4. The only exception to variables other than clean air that might have contributed significantly to the improvement

STAT5 deficiency does not affect the exp
STAT5 deficiency does not affect the expression of glutamine synthetase in mouse liver
✍ Theodorus B. M. Hakvoort; Jacqueline L. M. Vermeulen; Wouter H. Lamers πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 238 KB

In a recent study in HEPATOLOGY, Werth et al. 1 reported that STAT5 is a transcription factor, which binds to a section of the upstream regulatory region of the glutamine synthetase (GS) gene that, in transfection experiments, enhances reporter-gene expression. The next obvious step to further devel