Production and regeneration of protoplas
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E. S. Dias; E. F. AraΓΊjo; W. V. GuimarΓ£es; R. M. C. Muchovej
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Article
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1996
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Springer
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English
β 360 KB
Experiments were performed with the mycorrhizal fungus Suillus granulatus to define the parameters for production and regeneration of protoplasts. Protoplasts were released at frequencies between 1 and 3Γ10(7)/ml from mycelium 3 to 7 days old. The best osmotic stabilizer for protoplast release was M