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Factors affecting the production and regeneration of protoplasts: the case of the mycorrhizal fungus Tulasnella calospora from roots of orchids

✍ Scribed by Wang, Li-Kun ;Liu, Hong-Xia


Book ID
120924821
Publisher
Springer (Biomed Central Ltd.)
Year
2013
Tongue
English
Weight
468 KB
Volume
15
Category
Article
ISSN
2197-5620

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Production and regeneration of protoplas
✍ E. S. Dias; E. F. AraΓΊjo; W. V. GuimarΓ£es; R. M. C. Muchovej πŸ“‚ Article πŸ“… 1996 πŸ› Springer 🌐 English βš– 360 KB

Experiments were performed with the mycorrhizal fungus Suillus granulatus to define the parameters for production and regeneration of protoplasts. Protoplasts were released at frequencies between 1 and 3Γ—10(7)/ml from mycelium 3 to 7 days old. The best osmotic stabilizer for protoplast release was M