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Factors affecting the expression of foreign proteins inEscherichia coli

โœ Scribed by Bernard R. Glick; Gordon K. Whitney

Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
521 KB
Volume
1
Category
Article
ISSN
1476-5535

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โœ Shaojun Yang; Sven-Olof Enfors ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 439 KB

The effects of process conditions on proteolysis of three recombinant IgG-binding proteins ZT3,ZZT3 and protein A in E. coli were studied. SDS/PAGE shows that the relative amount of degradation intermediates of ZT3 in a shake flask cultivation is much higher than that in a bioreactor culture. The ra