Factors affecting sensitivity to EO9 in rodent and human tumour cells in vitro: DT-diaphorase activity and hypoxia
โ Scribed by Robertson, N.; Haigh, A.; Adams, G.E.; Stratford, I.J.
- Book ID
- 122744631
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 938 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0959-8049
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Studies with purified DT-diaphorase have shown that the enzyme is capable of catalyzing a two-electron reduction of the novel indoloquinone E 0 9 to a DNA-damaging alkylating species. The aim of this study was to determine to what extent DT-diaphorase may be involved in the metabolic activation of E
Using 4 human cancer cell lines, 4 tumors xenografted into nude mice, and I I fresh tumor specimens removed at surgery, we investigated the relevance of NAD(P)H:quinone oxidoreductase (DT-diaphorase, DTD) activity (nmoles/min/mg protein) to mitomycin C (MMC)-induced cytotoxicity. In culture cell lin