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Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting

✍ Scribed by Meunier, J.-R; Grimont, P.A.D


Book ID
120341994
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
659 KB
Volume
144
Category
Article
ISSN
0923-2508

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In this work, we have optimized random amplified polymorphic DNA (RAPD) for the use of human DNA in altering the concentration of the reaction components and the steps of the thermal profile in the polymerase chain reaction. By using two primers in every reaction and 2.5 U Taq DNA polymerase, we fou