Factors affecting a fluorometric assay of folic acid
โ Scribed by John R. Hazlett; Dane O. Kildsig
- Publisher
- John Wiley and Sons
- Year
- 1970
- Tongue
- English
- Weight
- 254 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0022-3549
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A new fluorometric assay method for quinolinic acid is introduced in this study. Quinolinic acid-hydrazine complex, a stable fluorescent compound, is formed after heating quinolinic acid with hydrazine at 215-220ยฐC for 2 min. Fluorescence excitation and emission maxima of the complex are at 285 and
Fluorescence of the thiobarbituric acid reaction product of periodatecleaved sialic acid is readily detected by excitation at 550 nm and analysis of emitted light at 570 nm. A fluorometric microassay is described which is 500-fold more sensitive than the usual spectrophotometric procedures and readi