✦ LIBER ✦

Fabricating of Acetylcholine Biosensor by a Layer-by-layer Deposition Technique for Determining Trichlorfon

✍ Scribed by Haibin Shi; Zixia Zhao; Zhao Song; Jiadong Huang; Yu Yang; Jun-ichi Anzai; Tetsuo Osa; Qiang Chen

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 168 KB
Volume: 17
Category: Article
ISSN: 1040-0397
DOI: 10.1002/elan.200403223

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

An acetylcholine (ACh) biosensor has been fabricated with bienzymes/poly(diallyldimethylammonium chloride) (PDDA) multilayer film‐modified platinum (Pt) electrodes by a layer‐by‐layer technique (LBL). The ACh biosensor was optimized and the properties are described. This ACh biosensor was used for the detection of organophosphate pesticide trichlorfon. The detection limits (found 0.001 μg/mL for trichlorfon) make it possible to detect the pollutants. This simple protocol of biosensor preparation, high sensitivity and stability are very promising for the determination of environmental pollutants in field conditions.

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