Extractive bioconversions in aqueous two-phase systems: Enzymatic hydrolysis of casein proteins
β Scribed by S. Mukataka; C. A. Haynes; J. M. Prausnitz; H. W. Blanch
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 803 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0006-3592
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β¦ Synopsis
Partition coefficients in poly(ethy1ene glycol)/dextran aqueous two-phase systems are reported for mixed-casein and its components, a, p and K casein. Rates of casein proteolysis by a-chymotrypsin and by trypsin are reported in single-phase and aqueous two-phase reactor systems. The advantages resulting from selective partitioning of substrates, enzymes, and products are examined in terms of relative volumetric reaction rates.
π SIMILAR VOLUMES
A new method for determination of t h e binodial of an aqueous two-phase system, using spectrophotometric measurements of the turbidity, is described in this article. The method is especially designed to characterize phase systems composed of polydisperse phase components. It gives information about
## Abstract **BACKGROUND:** Aqueous twoβphase extraction (ATPE) has many advantages as an efficient, inexpensive largeβscale liquidβliquid extraction technique for protein separation. However, the realization of ATPE as a protein separation technology at industrial scales is rather limited due to t