This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hand
Extraction of recombinant protein from Escherichia coli by using a novel cell autolysis activity of VanX
โ Scribed by Kamioka, Tetsuya; Sohya, Shihori; Wu, Nan; Maki, Tei; Matsuda, Tomoki; Ikegami, Takahisa; Nakamura, Haruki; Kuroda, Yutaka
- Book ID
- 123397710
- Publisher
- Elsevier Science
- Year
- 2013
- Tongue
- English
- Weight
- 761 KB
- Volume
- 439
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
In previous parts of this study we developed procedures for the high-efficiency chemical extraction of soluble and insoluble protein from intact Escherichia coli cells. Although high yields were obtained, extraction of recombinant protein directly from cytoplasmic inclusion bodies led to low product
A method is presented for the direct extraction of the recombinant protein Long-R 3 -IGF-I from inclusion bodies located in the cytoplasm of intact Escherichia coli cells. Chemical treatment with 6M urea, 3 mM EDTA, and 20 mM dithiothreitol (DTT) at pH 9.0 proved an effective combination for extract