A method is presented for the direct extraction of the recombinant protein Long-R 3 -IGF-I from inclusion bodies located in the cytoplasm of intact Escherichia coli cells. Chemical treatment with 6M urea, 3 mM EDTA, and 20 mM dithiothreitol (DTT) at pH 9.0 proved an effective combination for extract
โฆ LIBER โฆ
Direct chemical extraction of a recombinant viral coat protein from Escherichia coli at high cell density
โ Scribed by Woo-seok Choe; Anton P.J. Middelberg
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 194 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Chemical treatment of Escherichia coli.
โ
Robert J. Falconer; Brian K. O'Neill; Anton P. J. Middelberg
๐
Article
๐
1998
๐
John Wiley and Sons
๐
English
โ 161 KB
๐ 1 views
Chemical treatment of Escherichia coli:
โ
Robert J. Falconer; Brian K. O'Neill; Anton P. J. Middelberg
๐
Article
๐
1999
๐
John Wiley and Sons
๐
English
โ 308 KB
๐ 1 views
In previous parts of this study we developed procedures for the high-efficiency chemical extraction of soluble and insoluble protein from intact Escherichia coli cells. Although high yields were obtained, extraction of recombinant protein directly from cytoplasmic inclusion bodies led to low product
Framework for online optimization of rec
โ
Hee Jeong Chae; Matthew P. Delisa; Hyung Joon Cha; William A. Weigand; Govind Ra
๐
Article
๐
2000
๐
John Wiley and Sons
๐
English
โ 187 KB
๐ 2 views