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Direct chemical extraction of a recombinant viral coat protein from Escherichia coli at high cell density

โœ Scribed by Woo-seok Choe; Anton P.J. Middelberg


Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
194 KB
Volume
75
Category
Article
ISSN
0006-3592

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Chemical treatment of Escherichia coli.
โœ Robert J. Falconer; Brian K. O'Neill; Anton P. J. Middelberg ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 161 KB ๐Ÿ‘ 1 views

A method is presented for the direct extraction of the recombinant protein Long-R 3 -IGF-I from inclusion bodies located in the cytoplasm of intact Escherichia coli cells. Chemical treatment with 6M urea, 3 mM EDTA, and 20 mM dithiothreitol (DTT) at pH 9.0 proved an effective combination for extract

Chemical treatment of Escherichia coli:
โœ Robert J. Falconer; Brian K. O'Neill; Anton P. J. Middelberg ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 308 KB ๐Ÿ‘ 1 views

In previous parts of this study we developed procedures for the high-efficiency chemical extraction of soluble and insoluble protein from intact Escherichia coli cells. Although high yields were obtained, extraction of recombinant protein directly from cytoplasmic inclusion bodies led to low product