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Extraction of neuropeptides from joint tissue for quantitation by radioimmunoassay. A study in the rat

✍ Scribed by Mahmood Ahmed; Anders Bjurholm; Gopala Rao Srinivasan; Elvar Theodorsson; Andris Kreicbergs


Book ID
119142896
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
585 KB
Volume
15
Category
Article
ISSN
0196-9781

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A method for the extraction of acyl-CoA esters from tissue, and their subsequent analysis by HPLC is described. The lipids are removed by a two-phase extraction in a chloroform/methanol/water system. The long-chain acyl-CoA esters are extracted using methanol and a high salt concentration (2 M ammon