Quantitative extraction and estimation o
Quantitative extraction and estimation of intracellular nicotinamide nucleotides of Escherichia coli
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Esa-Matti Lilius; Veli-Matti Multanen; Veija Toivonen
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Article
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1979
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Elsevier Science
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English
โ 433 KB
The conditions of extraction of nicotinamide nucleotides from Escherichia coli were systematically investigated. The optimum pH for NAD+ and NADP+ appeared to be around 1.5 while that for NADH and NADPH was around 12.5. Seven minutes at 60ยฐC was chosen for the extraction of all four nucleotides. Add