Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells

✍ Scribed by Salvatore T. Butera; Victor L. Perez; Nora J. Besansky; Thomas M. Folks; Wing C. Chan; Bei-Yu Wu; Gary J. Nabel

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 718 KB
• Volume: 45
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240450410

✦ Synopsis

Abstract

In this report, we describe a human immunodeficiency virus type‐1 (HIV‐1)‐infected promyelocytic cell line, OM, derived from HL‐60 cells. Although the OM cell line was biologically cloned twice, the pattern of HIV‐1 expression during culture appeared analogous to a classical acute spreading infection and was inhibited by both azidothymidine and recombinant soluble CD4 treatment. The number of OM cells actually expressing HIV‐1 at the beginning of culture was 0%, reached a peak of nearly 100% at 6 weeks, and then fell to < 10% HIV‐1^+^ cells by 10 weeks. Clonal analysis of the surviving cells verified that stable HIV‐1^+^ OM cells resulted from the spreading infection. Southern analysis confirmed the transmission of HIV‐1 through these OM cultures and the occurrence of stable clones which resulted. The initial percentage of OM cells actually harboring the HIV‐1 genome was <0.1%, indicating nonfaithful transmission of an unintegrated HIV‐1 genome during clonal expansion. These results demonstrate that extrachromosomal HIV‐1 DNA can contribute to the spread of HIV‐1 infection and give rise to cells which have stably integrated HIV‐1 provirus.