Extracellular vesicles : methods and protocols

✍ Scribed by Jia, Shidong; Kuo, Winston Patrick

• Publisher: Humana Press ; Springer
• Year: 2017
• Tongue: English
• Leaves: 437
• Series: Methods in molecular biology 1660
• Category: Library

✦ Synopsis

This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling. The international group of expert scientists who have contributed to this collection provide a variety of different techniques related to the growing assortment of EV applications, given that at times using only one technique or two is insufficient to address the question at hand. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Comprehensive and practical, Extracellular Vesicles: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of EV functions and applications. Read more...

Abstract:

This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling. Read more...

✦ Table of Contents

Front Matter ....Pages i-xvi
Extracellular Vesicles: A Brief Overview and Its Role in Precision Medicine (Mingyi Shang, John S. Ji, Chao Song, Bao Jun Gao, Jason Gang Jin, Winston Patrick Kuo et al.)....Pages 1-14
Red Blood Cells: A Source of Extracellular Vesicles (Winston Patrick Kuo, John C. Tigges, Vasilis Toxavidis, Ionita Ghiran)....Pages 15-22
Front Matter ....Pages 23-23
Isolation of Extracellular Vesicles by Ultracentrifugation (Fatemeh Momen-Heravi)....Pages 25-32
Sequential Filtration: A Gentle Method for the Isolation of Functional Extracellular Vesicles (Mitja L. Heinemann, Jody Vykoukal)....Pages 33-41
Paper-Based for Isolation of Extracellular Vesicles (Yi-Hsing Hsiao, Chihchen Chen)....Pages 43-54
Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis (Cindy M. Yamamoto, Taku Murakami, Shu-Wing Ng)....Pages 55-63
Specific and Generic Isolation of Extracellular Vesicles with Magnetic Beads (Ketil W. Pedersen, Bente Kierulf, Axl Neurauter)....Pages 65-87
Front Matter ....Pages 89-89
Polymer-Based Purification of Extracellular Vesicles (Peter N. Brown, Hang Yin)....Pages 91-103
Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification (Richard Lobb, Andreas Möller)....Pages 105-110
Purification Protocols for Extracellular Vesicles (Rebecca E. Lane, Darren Korbie, Matt Trau, Michelle M. Hill)....Pages 111-130
Front Matter ....Pages 131-131
Characterization of Extracellular Vesicles by Surface Plasmon Resonance (Hyungsoon Im, Katherine Yang, Hakho Lee, Cesar M. Castro)....Pages 133-141
Extracellular Vesicle Isolation and Analysis by Western Blotting (Emma J. K. Kowal, Dmitry Ter-Ovanesyan, Aviv Regev, George M. Church)....Pages 143-152
Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis (Pauline Carnell-Morris, Dionne Tannetta, Agnieszka Siupa, Patrick Hole, Rebecca Dragovic)....Pages 153-173
Characterization of Extracellular Vesicles by Flow Cytometry (Virginia Camacho, Vasilis Toxavidis, John C. Tigges)....Pages 175-190
Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC) (Tao Huang, Jiang He)....Pages 191-199
Multi-Surface Antigen Staining of Larger Extracellular Vesicles (Veronika Lukacs-Kornek, Henrike Julich-Haertel, Sabine Katharina Urban, Miroslaw Kornek)....Pages 201-208
Microcapillary Chip-Based Extracellular Vesicle Profiling System (Takanori Akagi, Takanori Ichiki)....Pages 209-217
Front Matter ....Pages 219-219
Detection and Characterization of Extracellular Vesicles by Transmission and Cryo-Transmission Electron Microscopy (Petr Cizmar, Yuana Yuana)....Pages 221-232
Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy (Dmitry Ter-Ovanesyan, Emma J. K. Kowal, Aviv Regev, George M. Church, Emanuele Cocucci)....Pages 233-241
Front Matter ....Pages 243-243
In Vivo Tracking of Extracellular Vesicles in Mice Using Fusion Protein Comprising Lactadherin and Gaussia Luciferase (Yuki Takahashi, Makiya Nishikawa, Yoshinobu Takakura)....Pages 245-254
Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters (Anthony Yan-Tang Wu, Charles Pin-Kuang Lai)....Pages 255-265
Front Matter ....Pages 267-267
Extraction and Analysis of Extracellular Vesicle-Associated miRNAs Following Antibody-Based Extracellular Vesicle Capture from Plasma Samples (Davide Zocco, Natasa Zarovni)....Pages 269-285
Extracellular Vesicle miRNA Detection Using Molecular Beacons (Won Jong Rhee, Seunga Jeong)....Pages 287-294
Rapid Isolation of Extracellular Vesicles from Blood Plasma with Size-Exclusion Chromatography Followed by Mass Spectrometry-Based Proteomic Profiling (Simion Kreimer, Alexander R. Ivanov)....Pages 295-302
An Adaptable Polyethylene Glycol-Based Workflow for Proteomic Analysis of Extracellular Vesicles (Stephanie N. Hurwitz, David G. Meckes Jr.)....Pages 303-317
Front Matter ....Pages 319-319
Protocol for Exosome Isolation from Small Volume of Ovarian Follicular Fluid: Evaluation of Ultracentrifugation and Commercial Kits (Shlomit Kenigsberg, Brandon A. Wyse, Clifford L. Librach, Juliano C. da Silveira)....Pages 321-341
Isolation of Extracellular Vesicles in Saliva Using Density Gradient Ultracentrifugation (Kazuya Iwai, Satoshi Yamamoto, Mitsutaka Yoshida, Kiyotaka Shiba)....Pages 343-350
Isolation of Extracellular Vesicles from Breast Milk (Xin Wang)....Pages 351-353
An Integrated Double-Filtration Microfluidic Device for Detection of Extracellular Vesicles from Urine for Bladder Cancer Diagnosis (Li-Guo Liang, Ye-Feng Sheng, Sherry Zhou, Fatih Inci, Lanjuan Li, Utkan Demirci et al.)....Pages 355-364
Front Matter ....Pages 365-365
Electric Field-Induced Disruption and Releasing Viable Content from Extracellular Vesicles (Chris Wang, Austin Wang, Fang Wei, David T. W. Wong, Michael Tu)....Pages 367-376
Production and Characterization of Extracellular Vesicles in Malaria (Smart Mbagwu, Michael Walch, Luis Filgueira, Pierre-Yves Mantel)....Pages 377-388
Isolation of Extracellular Vesicles from Stem Cells (Zixin Chen, Yongjun Li, Hong Yu, Yan Shen, Chengwei Ju, Genshan Ma et al.)....Pages 389-394
Front Matter ....Pages 395-395
The Use of Peripheral Extracellular Vesicles for Identification of Molecular Biomarkers in a Solid Tumor Mouse Model (Noemí García-Romero, Gorjana Rackov, Cristobal Belda-Iniesta, Ángel Ayuso-Sacido)....Pages 397-406
Front Matter ....Pages 407-407
Therapeutic Applications of Extracellular Vesicles: Perspectives from Newborn Medicine (Gareth R. Willis, Stella Kourembanas, S. Alex Mitsialis)....Pages 409-432
Therapeutic Use of Tumor Cell-Derived Extracellular Vesicles (Jing Liu, Jingwei Ma, Ke Tang, Bo Huang)....Pages 433-440
Erratum to: An Adaptable Polyethylene Glycol-Based Workflow for Proteomic Analysis of Extracellular Vesicles (Stephanie N. Hurwitz, David G. Meckes Jr.)....Pages E1-E1
Back Matter ....Pages 441-446

✦ Subjects

Ribonucleases -- Laboratory manuals;Exosomes;Secretory Vesicles;Cell-Derived Microparticles;Ribonucleases