The Extracellular Matrix: Methods and Protocols

✍ Scribed by Davide Vigetti, Achilleas D. Theocharis

• Publisher: Springer New York;Humana Press
• Year: 2019
• Tongue: English
• Leaves: 282
• Series: Methods in Molecular Biology 1952
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology.

✦ Table of Contents

Front Matter ....Pages i-xii
The Complex Interplay Between Extracellular Matrix and Cells in Tissues (Dimitra Manou, Ilaria Caon, Panagiotis Bouris, Irene-Eva Triantaphyllidou, Cristina Giaroni, Alberto Passi et al.)....Pages 1-20
Method for Studying ECM Expression: In Situ RT-PCR (Elena Caravà, Cristiana Marcozzi, Barbara Bartolini, Marcella Reguzzoni, Paola Moretto, Ilaria Caon et al.)....Pages 21-31
Visualizing the Supramolecular Assembly of Collagen (Mario Raspanti, Marcella Reguzzoni, Petra Rita Basso, Marina Protasoni, Désirée Martini)....Pages 33-44
Steady-State and Pulse-Chase Analyses of Fibrillar Collagen (Antonella Forlino, Francesca Tonelli, Roberta Besio)....Pages 45-53
Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates (Mariana P. Stelling, Ananda A. de Bento, Philippe Caloba, Eduardo Vilanova, Mauro S. G. Pavão)....Pages 55-70
Analysis of Proteoglycan Synthesis and Secretion in Cell Culture Systems (Chiara Paganini, Rossella Costantini, Antonio Rossi)....Pages 71-80
Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples (Sanna Oikari, Markku I. Tammi)....Pages 81-89
Methods for Hyaluronan Molecular Mass Determination by Agarose Gel Electrophoresis (Mary K. Cowman)....Pages 91-102
Hyaluronan Pericellular Matrix: Particle Exclusion Assay (Melanie A. Simpson)....Pages 103-110
Determination of Cell-Surface Hyaluronan Through Flow Cytometry (Daiana L. Vitale, Fiorella M. Spinelli, Laura Alaniz)....Pages 111-116
Method for Detecting Hyaluronan in Isolated Myenteric Plexus Ganglia of Adult Rat Small Intestine (Michela Bistoletti, Paola Moretto, Cristina Giaroni)....Pages 117-125
Analyzing Hyaluronidases in Biological Fluids (Christos Velesiotis, Stella Vasileiou, Demitrios H. Vynios)....Pages 127-142
Method for Studying the Physical Effect of Extracellular Matrix on Voltage-Dependent Ion Channel Gating (Eleonora Solari, Andrea Moriondo)....Pages 143-156
Methods for Monitoring Matrix-Induced Autophagy (Carolyn Chen, Aastha Kapoor, Renato V. Iozzo)....Pages 157-191
Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography (Elin Hadler-Olsen, Jan-Olof Winberg)....Pages 193-199
Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography (Elin Hadler-Olsen, Jan-Olof Winberg)....Pages 201-210
In Vitro Spheroid Sprouting Assay of Angiogenesis (Fatema Tuz Zahra, Efrossini Choleva, Md Sanaullah Sajib, Evangelia Papadimitriou, Constantinos M. Mikelis)....Pages 211-218
Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis (Pinelopi Kastana, Fatema Tuz Zahra, Despoina Ntenekou, Stamatiki Katraki-Pavlou, Dimitris Beis, Michail S. Lionakis et al.)....Pages 219-232
Exosomes from Cell Culture-Conditioned Medium: Isolation by Ultracentrifugation and Characterization (Anurag Purushothaman)....Pages 233-244
Preparation and Characterization of Tissue Surrogates Rich in Extracellular Matrix Using the Principles of Macromolecular Crowding (Adrian Djalali-Cuevas, Sergio Garnica-Galvez, Andrea Rampin, Diana Gaspar, Ioannis Skoufos, Athina Tzora et al.)....Pages 245-259
Novel Approaches for Extracellular Matrix Targeting in Disease Treatment (Nikolaos A. Afratis, Irit Sagi)....Pages 261-275
Back Matter ....Pages 277-278

✦ Subjects

Life Sciences; Cell Biology; Biochemistry, general