Extent and high frequency of a short conversion between the human Aγ and Gγ fetal globin genes

✍ Scribed by J. Starck; R. Bouhass; F. Morlé; J. Godet

• Book ID: 104665422
• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 806 KB
• Volume: 84
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00208937

✦ Synopsis

Southern blotting and DNA sequencing after polymerase chain reaction (PCR) amplification provide evidence for the frequent occurrence (in 7 out of 24 chromosomes) of a short conversion G gamma----A gamma in the 3' end of the human fetal A gamma globin gene. This short conversion is characterized by the presence, 3 nucleotides downstream from the termination codon of the A gamma gene, of the TCAC sequence that is normally present at the equivalent position at the 3' end of the G gamma gene; it is therefore identical to a conversion already described. Interestingly, we have found that this conversion is associated with the presence of the HindIII polymorphic restriction site in the A gamma IVS2, occupying an equivalent position in both the G gamma and A gamma genes. Our observations strengthen the hypothesis that the presence of the HindIII polymorphic restriction site in A gamma IVS2 and the presence of the sequence TCAC at the 3' end of the A gamma gene might be the result of a single conversion event.