Expression Vectors for the Rapid Purification of Recombinant Proteins inBacillus subtilis
โ Scribed by Hoang Duc Nguyen; Trang Thi Phuong Phan; Wolfgang Schumann
- Publisher
- Springer
- Year
- 2007
- Tongue
- English
- Weight
- 168 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0343-8651
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A system has been developed for synthesis and rapid purification of recombinant polypeptides expressed in frame with glutathione S-transferase (D. B. Smith and K. S. Johnson, 1988, Gene 67, 31-40). Expressed fusion proteins are purified from bacterial extracts by glutathione-agarose affinity chromat
## Abstract We have combined Invitrogen's Gatewayยฎ cloning technology with selfโcleaving purification tags to generate a new system for rapid production of recombinant protein products. To accomplish this, we engineered our previously reported ฮIโCM cleaving intein to include a Gateway cloning reco