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Expression screening, protein purification and NMR analysis of human protein domains for structural genomics

โœ Scribed by G.E. Folkers; B.N.M. van Buuren; R. Kaptein


Book ID
111596475
Publisher
Springer Netherlands
Year
2004
Tongue
English
Weight
560 KB
Volume
5
Category
Article
ISSN
1345-711X

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A simple and rapid method based on 15N labeling and 1H-15N heteronuclear single quantum coherence spectroscopy is presented to directly assess the structural integrity of overexpressed proteins in crude Escherichia coli extracts without the need for any purification. The method is demonstrated using