Expression and Purification of the Extracellular Domain of Human Myelin Protein Zero
โ Scribed by Jeremy P. Bond; Raul A. Saavedra; Daniel A. Kirschner
- Book ID
- 115645935
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 334 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1046-5928
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Myelin basic protein (MBP) occurs in multiple forms. Three of these isoforms from human MBP (HMBP) have been highly purified. HMBP, component 1 (18.5 kDa HMBP-l), was purified by ion-exchange chromatography at pH 10.6 in 2 M urea. During this ion-exchange chromatography, a fraction (Fraction 3), whi
The human brain contains four isoforms of myelin basic protein (MBP), previously identified by cDNA cloning. We have now isolated and characterized genomic clones encoding the human MBP gene. The gene is 45 kb in extent and consists of seven exons. Alternative splicing of the primary MBP transcript