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Expression, purification, crystallization, and preliminary X-ray analysis of the N-terminal domain of Escherichia coli adenylyl transferase

✍ Scribed by Yibin Xu; Daying Wen; Paula Clancy; Paul D. Carr; David L. Ollis; Subhash G. Vasudevan


Book ID
116827766
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
202 KB
Volume
34
Category
Article
ISSN
1046-5928

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Crystallization and preliminary x-ray an
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## Peptidyl-tRNA hydrolase from Escherichia coli, a monomer of 21 kDa, was overexpressed from its cloned gene pth and crystallized by using polyethylene glycol as precipitant. The crystals are orthorhombic and have unit cell parameters a 5 47.24 Γ…, b 5 63.59 Γ…, and c 5 62.57 Γ…. They belong to spac