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Expression, purification, crystallization, and preliminary X-ray analysis of recombinant human saposin B

✍ Scribed by Victoria E. Ahn; Kym F. Faull; Julian P. Whitelegge; Jason Higginson; Arvan L. Fluharty; Gilbert G. Privé

Book ID: 117702604
Publisher: Elsevier Science
Year: 2003
Tongue: English
Weight: 242 KB
Volume: 27
Category: Article
ISSN: 1046-5928
DOI: 10.1016/s1046-5928(02)00597-1

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Diffraction quality crystals of recombinant staphylokinase (STAR) have been grown by the hanging drop vapor diffusion technique from a solution containing MgCl 2 , Tris buffer (pH 8.5), and polyethylene glycol 4000. The crystals belong to the monoclinic space group C2 with unit cell dimensions a ‫؍‬