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Expression, purification, and immobilization of His-taggedd-amino acid oxidase ofTrigonopsis variabilisinPichia pastoris

โœ Scribed by Huabao Zheng; Xiaolan Wang; Jun Chen; Ke Zhu; Yuhua Zhao; Yunliu Yang; Sheng Yang; Weihong Jiang

Publisher
Springer
Year
2006
Tongue
English
Weight
183 KB
Volume
70
Category
Article
ISSN
1432-0614

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Immobilized glucose oxidase and d-amino acid oxidase: A convenient method for the purification of flavin adenine dinucleotide and its analogs
โœ Vincent Massey; Loren D. Mendelsohn ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 330 KB

The preparation of flavin adenine dinucleotide-aftinity columns employing glucose oxidase and D-amino acid oxidase covalently linked to Sepharose-4B is described. Both immobilized enzymes have very good long-term stabilities, retaining at least half of their original flavin adenine dinucleotide-bind