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Expression, Purification, and Crystallization of Two Isozymes of 6-Phosphoglucose Isomerase ofBacillus stearothermophilus

✍ Scribed by Chwan-Deng Hsiao; Chia-Cheng Chou; Yi-Yuong Hsiao; Yuh-Ju Sun; Menghsiao Meng

Book ID
115633862
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
188 KB
Volume
120
Category
Article
ISSN
1047-8477

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Esterase-6 (EST 6; carboxylic-ester hydrolase; EC 3.1.1.1) from Drosophila melanogaster was purified to homogenity. Purified enzyme occurs as two closely moving isozymes, slow (EST 6S) and fast (EST 6F), on native polyacrylamide gel electrophoresis. Except for slight differences in their mobility, t