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Cloning, expression, and purification of Bacillus stearothermophilus DNA primase and crystallization of the zinc-binding domain

โœ Scribed by Hu Pan; Louise E. Bird; Dale B. Wigley


Book ID
114148491
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
476 KB
Volume
1444
Category
Article
ISSN
0167-4781

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A 124-residue N-terminal fragment corresponding to the DNA-binding domain of the Saccharomyces cerevisae cell-cycle transcription factor MBP-1 has been expressed with a hexahistidine affinity tag in E. coli and purified to apparent homogeneity. Crystals have been grown using PEG 3350 as precipitant