Expression of theRuminococcus flavefacienscellodextrinase gene inSaccharomyces cerevisiae

✍ Scribed by Pierre Rensburg; Willem H. Zyl; Isak S. Pretorius

• Publisher: Springer Netherlands
• Year: 1995
• Tongue: English
• Weight: 420 KB
• Volume: 17
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00132014

✦ Synopsis

A recombinant strain of Saccharomyces cerevtslae secreting bacterial cellodextrinase was constructed. The Rummococcus flavefaclens cellodextrinase gene (celA) was inserted between a yeast expression-secretion cassette and yeast gene terminator, and cloned into a yeast-centromeric shuttle vector. Enzyme assays revealed growth-associated production of biologically active cellodextrinase by S. cerewstae transformants.