A recombinant strain of Saccharomyces cerevtslae secreting bacterial cellodextrinase was constructed. The Rummococcus flavefaclens cellodextrinase gene (celA) was inserted between a yeast expression-secretion cassette and yeast gene terminator, and cloned into a yeast-centromeric shuttle vector. Enz
โฆ LIBER โฆ
Expression of theKlebsiella pneumoniaepullulanase-encoding gene inSaccharomyces cerevisiae
โ Scribed by Bernard J. H. Janse; Isak S. Pretorius
- Publisher
- Springer-Verlag
- Year
- 1993
- Tongue
- English
- Weight
- 866 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0172-8083
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Genetic engineering of wine yeast strains requires the identification of gene promoters specifically activated under wine processing conditions. In this study, transcriptional activation of specific genes was followed during the time course of wine fermentation by quantifying mRNA levels in a haploi