Expression of the HTLV-III envelope gene by a recombinant vaccinia virus
β Scribed by Chakrabarti, Sekhar; Robert-Guroff, Marjorie; Wong-Staal, Flossie; Gallo, Robert C.; Moss, Bernard
- Book ID
- 109743311
- Publisher
- Nature Publishing Group
- Year
- 1986
- Tongue
- English
- Weight
- 410 KB
- Volume
- 320
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/320535a0
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The complete human T-cell leukemia virus type I (HTLV-I) env gene was inserted into an expression cassette containing the adenovirus 5 major late promoter (Ad5-MLP). Recombinant Ad5-HTLV-I-env was obtained by homologous recombination in 293 cells simultaneously transfected by the expression cassette
## Abstract Two groups of 3 rabbits each were immunized with either recombinant vaccinia virus, WRβSFB5env, carrying the human Tβcell lymphotropic virus type I (HTLVβ1) __env__ gene at the site of the hemagglutinin gene of the WR strain, or control vaccinia virus, HAβWR, lacking the functional hema