Sera from transgenic mice (TM) carrying human genes of alpha 1-acid glycoprotein (orosomucoid or ORM) have been analyzed by isoelectrofocusing and subsequent immunoblotting with antihuman ORM antibodies. With this technique it is possible to reveal selectively the human protein secreted in the TM se
Expression of the genetic variants of human alpha-1-acid glycoprotein in cancer
✍ Scribed by Jean-Claude Duché; Saïk Urien; Nicolas Simon; Emmanuelle Malaurie; Isabelle Monnet; Jérôme Barré
- Book ID
- 108313488
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 185 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0009-9120
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Human alpha(1)-acid glycoprotein (AGP), a major carrier of many basic drugs in circulation, consists of at least two genetic variants, namely A and F1\*S variant. Interestingly, the variants of AGP have different drug-binding properties. The purpose of this study was to identify the amino acid resid
A novel probe was developed to measure drug association with the F1\*S variant of the human serum protein alpha 1-acid glycoprotein (AGP). The molecule 2hydroxy-3,5-diiodo-N-[2(diethylamino)ethyl]benzamide (DEDIC) binds to AGP, quenching its native ¯uorescence. This quenching was ®tted to a two-site