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Use of transgenic mice for the characterization of human alpha 1-acid glycoprotein (orosomucoid) variants

✍ Scribed by L. Tomei; C. B. Eap; P. Baumann; L. Dente

Publisher
Springer
Year
1989
Tongue
English
Weight
259 KB
Volume
84
Category
Article
ISSN
0340-6717

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✦ Synopsis

Sera from transgenic mice (TM) carrying human genes of alpha 1-acid glycoprotein (orosomucoid or ORM) have been analyzed by isoelectrofocusing and subsequent immunoblotting with antihuman ORM antibodies. With this technique it is possible to reveal selectively the human protein secreted in the TM sera. Orosomucoid bands present in TM sera have been compared with those of the most common human ORM phenotypes to correlate the products of specific genes to previously identified genetic variants. In this paper, we report the identification of the genes encoding for variants ORM1 F1 and ORM2 A, which are genes AGP-A and AGP-B/B' respectively. The nucleotide sequences of these genes are known; therefore a direct correlation between variants and specific amino acid sequences can be established.

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