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Expression of the Chlamydia trachomatis major outer membrane protein-encoding gene in Escherichia coli: role of the 3′ end in mRNA stability

✍ Scribed by Kaul, Ravi; Duncan, Matthew J.J.; Guest, James; Wenman, Wanda M.


Book ID
120544774
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
946 KB
Volume
87
Category
Article
ISSN
0378-1119

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To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat