Expression of the active human and duck hepatitis B virus polymerases in heterologous system of Pichia methanolica
β Scribed by Juhyun Choi; Eunice E. Kim; Young In Park; Ye Sun Han
- Book ID
- 114144414
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 380 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0166-3542
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Replication of hepadnaviruses involves a viral DNA polymerase containing both a DNA-dependent and an RNA dependent activity. This polymerase is a potential target for chemotherapy against hepatitis B. We have used human hepatitis B virus DNA-dependent DNA polymerase from human serum and duck hepatit
## Abstract Primary duck hepatocyte (PDH) cultures were established from ducklings congenitally infected with the duck hepatitis B virus (DHBV), plated onto feeder cell layers of irradiated human embryonic lung fibroblasts, and observed for 2 to 3 weeks. This system permitted the survival of the PD