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Expression of tetracycline resistance in pBR322 derivatives reduces the reproductive fitness of plasmid-containing Escherichia coli

✍ Scribed by Lee, Sam Whan; Edlin, Gordon

Book ID
122503295
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
698 KB
Volume
39
Category
Article
ISSN
0378-1119

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Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivatives
✍ Lopilato, Jane ;Bortner, Scott ;Beckwith, Jon πŸ“‚ Article πŸ“… 1986 πŸ› Springer 🌐 English βš– 711 KB

We describe mutants of Escherichia coli that decrease the plasmid copy number of pBR322 derivatives. One mutant was partially characterized genetically and its mutation, designated pcnB for plasmid copy number, was mapped to approximately 3 rain on the E. coli chromosome. This locus is distinct from