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Expression of the ptsH+ gene of Escherichia coli cloned on plasmid pBR322: A convenient means for obtaining the histidine-containing carrier protein HPr

✍ Scribed by Lynne G. Lee; Paul Britton; Francisco Parra; Albert Boronat; Hans Kornberg

Book ID: 115913987
Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 514 KB
Volume: 149
Category: Article
ISSN: 0014-5793
DOI: 10.1016/0014-5793(82)81119-8

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The btuB gene of Escherichia coli codes for a protein (BtuB) located in the outer membrane. BtuB is the receptor for vitamin B12 (cyanocobalamin). We have cloned the btuB gene into pUC8 using transposon Tn5 as the marker to first isolate several parts of the relevant DNA fragment from the specialize