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Expression of proteins using the third domain of the Escherichia coli periplasmic-protein TolA as a fusion partner

✍ Scribed by Gregor Anderluh; Isa Gökçe; Jeremy H Lakey

Book ID
117702665
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
353 KB
Volume
28
Category
Article
ISSN
1046-5928

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We have constructed three plasmid vectors for the expression of green fluorescent protein (GFP) fusion proteins using the following motif: (His) 6 -GFP-EK-X, where X represents chloramphenicol acetyl-transferase (CAT), human interleukin-2 (hIL-2), and organophosphorous hydrolase (OPH), respectively,