Expression of p21waf1/cip1, mdm2 and p53 in vivo: analysis of cytological preparations
β Scribed by S. P. DOWELL; E. McGOOGAN; S. M. PICKSLEY; W. S. EL-DEIRY; B. VOGELSTEIN; P. A. HALL
- Book ID
- 108705906
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 1005 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0956-5507
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Data are non-existent regarding coincidental alterations in the expression of p53 and its downstream target genes MDM2 and p21 Waf1/Cip1 in gastric carcinogenesis. An immunohistochemical study was therefore performed to examine the interrelationships of p53, MDM2, and p21 Waf1/Cip1 expression in a s
RNase H 0 reverse transcriptase (GIBCO-BRL, Gaithersburg, MD). ase chain reaction; SSCP, single-strand conformation polymorphism; mRNA, messenger p21 WAF1/CIP1 messenger RNA (mRNA) was amplified with the follow-RNA; cdk, cyclin dependent kinase.
the pancreas were immunostained for p53 and p21. Nuclear expression was scored as absent, focal (Γ΅10%), moderate (10 -50%), or strong or diffuse