Expression of matrilysin in vascular endothelial cells adjacent to matrilysin-producing tumors

✍ Scribed by Yoji Nagashima; Satoshi Hasegawa; Naohiko Koshikawa; Atsuko Taki; Yasushi Ichikawa; Hitoshi Kitamura; Kazuaki Misugi; Yasunori Kihira; Yuhsi Matuo; Hidetaro Yasumitsu; Kaoru Miyazaki

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: French
• Weight: 232 KB
• Volume: 72
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19970729)72:3<441::aid-ijc11>3.0.co;2-f

✦ Synopsis

Matrilysin is believed to play important roles in tumor progression and metastasis. In the present study, we analyzed matrilysin-producing cells in various human cancer tissues by immunohistochemistry and in situ hybridization. Tumor cells in colorectal carcinomas, pancreatic carcinomas, transitionalcell carcinomas of the kidney and small-cell lung carcinomas were frequently positive for matrilysin. In addition, we found that endothelial cells of arterioles and venules adjacent to matrilysin-positive tumors expressed matrilysin mRNA and protein. The endothelial cells adjacent to matrilysin-negative tumors and those in normal tissues were negative for matrilysin. Furthermore, analyses by casein zymography, Western blotting and RT-PCR showed that matrilysin was weakly expressed by cultured human umbilical vein endothelial cells. Our results suggest that the expression of matrilysin in vascular endothelial cells and in tumor cells may be regulated by common soluble factors, and that endothelial cell-derived matrilysin may contribute to tumor angiogenesis and tumor metastasis.